Testing Antimicrobial Activity Using The Time Kill Method
The Time Kill Test, or Time Kill Analysis, is carried out to evaluate an antimicrobial test material or antibiotic and assesses the in vitro reduction of a microbial population of test organisms after exposure to a test material.
The Time Kill Analysis measures the change in a population of microorganisms within a specified sampling time when after exposure to antimicrobial test materials in vitro. The test article or its dilution is brought into contact with a known population of microorganisms for a specified period of time at a specified temperature. The test article is then neutralized at the target sampling time and the surviving organisms are enumerated. The percent and/or log10 reduction from either an initial microbial population or test blank is calculated.
Several options for organism selection and growth, inoculum preparation, sampling times and temperatures can be requested.