Testing Antimicrobial Activity Using The Time Kill Method

Pacific BioLabs has extensive experience with time kill assays for pharmaceuticals, disinfectants and other products. Our microbiology team can help with protocol development and tailor the study based on the client’s needs. We will perform initial suitability to determine whether the sample has inherent antimicrobial properties that will inhibit the growth of microorganisms during the test.

The Time Kill Test

The Time Kill Test, or Time Kill Analysis, is carried out to evaluate an antimicrobial test material and assesses the in vitro reduction of a microbial population of test organisms after exposure to a test material.

The Time Kill Analysis measures the change in a population of microorganisms within a specified sampling time after exposure to an antimicrobial test materials in vitro. The test article or its dilution is brought into contact with a known population of microorganisms for a specified period of time at a specified temperature. The test article is then neutralized at the target sampling time and the surviving organisms are enumerated. The percent and/or log10 reduction from either an initial microbial population or test blank is calculated.

Several options for organism selection and growth, inoculum/suspension preparation, sampling times and temperatures can be requested. Our microbiology team has performed complex time kill studies involving various organisms and time points. Our microbiology team will be happy to help you with your time kill study.

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